Table 1 Elution program for determination of lactose
Time (min) | Flow rate (mL min−1) | Eluent ‘A’ (%) | Eluent ‘B’ (%) | Eluent ‘C’ (%) |
|---|---|---|---|---|
0.0 | 0.5 | 2.0 | 98.0 | 0.0 |
1.0 | 0.5 | 2.0 | 98.0 | 0.0 |
12.0 | 0.5 | 5.0 | 95.0 | 0.0 |
21.0 | 0.5 | 22.4 | 65.6 | 12.0 |
21.1 | 0.5 | 0.0 | 0.0 | 100.0 |
26.0 | 0.5 | 0.0 | 0.0 | 100.0 |
26.1 | 0.5 | 100.0 | 0.0 | 0.0 |
31.0 | 0.5 | 100.0 | 0.0 | 0.0 |
31.1 | 0.5 | 2.0 | 98.0 | 0.0 |
37.0 | 0.5 | 2.0 | 98.0 | 0.0 |
