Fig. 4 | Laboratory Investigation

Fig. 4

From: Maresin 1 attenuates mitochondrial dysfunction through the ALX/cAMP/ROS pathway in the cecal ligation and puncture mouse model and sepsis patients

Fig. 4

The beneficial effects of MaR1 were abrogated by BOC-2 in CLP-induced sepsis. MaR1 (100 ng/mice, i.p.) in the presence or absence of BOC-2 (600 ng/kg, i.p.) was administered to C57BL/6 mice after surgery and survival rate was observed for 8 days. The control groups for Fig. 1 and Fig. 4 were the same set of animals. (a). The lung tissues were collected 24 h after CLP and the effect of MaR1 or BOC-2 was assessed by histology in H&E-stained sections (original magnification ×100) (b). Lung injury scores (b) were recorded from 0 (no damage) to 16 (maximum damage) according to the criteria described in Materials and Methods. Part of the right lung was homogenized from individual mice and centrifuged, then the tissue level of TNF-α in the resulting supernatants was determined using ELISA kit. (c) Data are presented as mean ± SEM. n = 6–12. **p < 0.01 vs. CLP group. ##p < 0.01 vs. CLP+MaR1 group, $$p < 0.01 vs. SHAM group

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