Fig. 4

Ubc9 Tg animals are protected from injury due to blunted inflammatory responses after intestinal I/R. a Sections from wild-type or Ubc9Tg animals undergoing 45 min of ischemia, and either 1, 3, or 6 h of reperfusion were stained by H&E and scored using the Chiu intestinal I/R score. n = 6/condition. b Plasma levels of gavaged FITC dextran (in µg FITC dextran/ml plasma) as a measure of intestinal permeability from wild-type or Ubc9Tg animals after 45 min of ischemia and either 3 or 6 h of reperfusion. n = 4/condition. c Myeloperoxidase assay of intestinal samples collected after 45 min and 3 or 6 h of reperfusion. n = 6/condition, *p ≤ 0.05. Microarray analysis of transcriptional responses in Ubc9Tg (Tg) vs. wild-type littermates after I/R. d Covariance-based principal component analysis (PCA) scatter plot depicts the Ubc9Tg vs. wild-type sample-to-sample relationships by time using the union set of 5487 genes identified differentially expressed for at least one possible sample type comparison. e Venn diagram summarizes the number of differential expressed genes observed between Ubc9Tg vs wild type that are either exclusive by time or shared across time. The top 10 significantly enriched f functions and g pathways returned from the Ingenuity Pathway Analysis (IPA) tool when provided the list of 474 genes differentially expressed between Ubc9Tg and wild type subjected to 45 min of ischemia and 6 h of reperfusion. n = 4/condition