Fig. 5

Modulation of villus-tip epithelial responses in Ubc9Tg animals after I/R. Intestinal epithelial villus-tip epithelia from ischemic regions of wild type and Ubc9Tg (45 minutes of ischemia and 3 h of reperfusion) were isolated by laser microdissection and capture and RNA was isolated to perform a cytokine targeted PCR array. a Scatter and b Volcano blot outline the distribution of regulated genes; significantly up- and downregulated genes are listed in c. d IL17A protein levels in non-ischemic small intestinal loops (NI) and loops subjected to ischemia/reperfusion (IR; 45 min ischemia and 6 h or reperfusion). Quantification was performed by densitometry and is represented as the ratio of IL17A to β-Actin band intensity. n = 5; *p ≤ 0.05