Fig. 6

SUMO1 knockout (SUMO1-KO) animals display a blunted inflammatory response to intestinal I/R injury. a H&E-stained section from wild-type and SUMO1- KO animals were scored using the Chiu intestinal I/R score. b Plasma levels of gavaged FITC dextran (in µg FITC dextran/ml plasma) from wild type or SUMO1-KO animals. c Myeloperoxidase assay. d–f Representative images from Naphthol AS-D Chloroacetate esterase staining of intestinal sections from wild-type (d,e) or SUMO1-KO animals (f) after I/R injury. Images were taken from relatively intact sections adjacent to areas of significant injury. Arrows denote infiltrating neutrophils. Magnification × 400. g qPCR from post-ischemic small intestines in SUMO-1 KO and WT mice. Graphs represent fold change over sham-treated wild-type animals and notes the fold difference of response between SUMO1-KO and WT, consistent with Table 1. Assays were performed on wild-type (n = 4) or SUMO1-KO (n = 5) animals subjected 45 min of ischemia and 6 h of reperfusion. *p ≤ 0.05