Fig. 3 | Laboratory Investigation

Fig. 3

From: SOCS1-targeted therapy ameliorates renal and vascular oxidative stress in diabetes via STAT1 and PI3K inhibition

Fig. 3

Effect of SOCS1-based therapies on the expression of Nox subunits in diabetic mice. a, b Gene expression analysis of Nox isoforms in kidney, aorta, and peritoneal macrophages (MΦ) from diabetic mice treated with SOCS1-recombinant adenovirus (a) and SOCS1 peptidomimetic (b). Real-time PCR values were normalized to 18S rRNA and expressed as relative mRNA level compared with non-diabetic mice (horizontal dotted lines). c Representative micrograph showing immunodetection of Nox1 and Nox4 isoforms in kidney (scale bar, 20 μm) and aorta (scale bar, 20 μm; L lumen) of diabetic mice at early and late disease checkpoints. d Quantification of positive cells in kidney (GLOM glomeruli, TUB-INT tubulointerstitium) and aorta. Bars are the mean ± SEM of 6–10 mice per group. *P < 0.05 vs Control, #P < 0.05 vs Ad-null, $P < 0.05 vs respective Veh (early or late model), and &P < 0.05 vs Mut

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