Fig. 5
SOCS1 prevents Nox-mediated ROS generation in vitro. a Intracellular detection of O2•− in VSMC and MCT pretreated with SOCS1 peptide (100 μg/mL, 90 min) before stimulation with either cytokines (103 U/mL IFNγ plus 102 U/mL IL6, 1 h) or HG (30 mmol/L d-glucose, 24 h). Representative fluorescence images (red, DHE staining; blue, DAPI nuclear staining) and summary of quantification are shown. b Time-response effect of SOCS1 peptide on Nox-dependent ROS generation in VSMC, MCT and BMDM was assessed by lucigenin chemiluminiscence assay. c Dose-dependent curve of SOCS1 peptide on HG-stimulated Nox activity in MCT. d Effect of adenovirus-mediated SOCS1 overexpression on Nox activity in cytokine-stimulated VSMC and BMDM. Data expressed as fold increases over basal conditions (arbitrarily set to 1) or lucigenin relative units are the mean ± SEM of 3–6 independent experiments. *P < 0.05 vs Basal, #P < 0.05 vs Cytokines, and $P < 0.05 vs HG
