Fig. 6 | Laboratory Investigation

Fig. 6

From: SOCS1-targeted therapy ameliorates renal and vascular oxidative stress in diabetes via STAT1 and PI3K inhibition

Fig. 6

SOCS1 blunts assembly and expression of Nox subunits in cultured cells. a Effect of SOCS1 peptide on cytokine-induced membrane translocation of Nox1A and NoxO1 regulatory subunits in VSMC. Membrane and cytosolic fractions were obtained after 2 h of stimulation and then immunoblotted for NoxA1, NoxO1, Nox1, α-tubulin (cytosolic loading control), and EGFR (membrane loading control). Shown are representative immunoblots and the summary of normalized quantification. b, c Real-time PCR analysis of Nox subunits (normalized to 18S) in cytokine-stimulated VSMC (b) and BMDM (c) over time. d Nox mRNA expression levels (normalized to 18S) in MCT at 24 h of HG incubation. Data expressed as fold increases over basal conditions are the mean ± SEM of 4–7 independent experiments. *P < 0.05 vs Basal, #P < 0.05 vs Cytokines, and $P < 0.05 vs HG

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