Fig. 1 | Laboratory Investigation

Fig. 1

From: ZBTB46 is a shear-sensitive transcription factor inhibiting endothelial cell proliferation via gene expression regulation of cell cycle proteins

Fig. 1

ZBTB46 mRNA is expressed in mouse arterial endothelial cells in vivo and is downregulated in areas exposed to disturbed flow where EC proliferation is increased, and correlates with CDKN1A (p21) gene expression. We performed a partial ligation procedure in C57Bl/6 mice which produces a disturbed flow profile in the LCA inducing endothelial cell activation, while retaining a unidirectional physiologic flow in the RCA where ECs are quiescent. EC-enriched RNA were isolated 2 days post ligation from the LCA and RCA, and ZBTB46 and CDKN1A (p21) mRNA expression in the LCA were compared to the RCA as control. ZBTB46 mRNA expression was also compared in the greater curvature (GC) vs. the lesser curvature (LC) of the aortic arch. a, c Endothelial cell ZBTB46 and CDKN1A(p21) mRNA expression in right common carotid artery (RCA) vs. left common carotid artery (LCA, partially ligated) showing downregulation of ZBTB46 and CDKN1A in the LCA. *P < 0.05, ***P < 0.01 two-tailed t-test, n = 9. b ZBTB46 mRNA expression in the greater curvature (GC) vs. the lesser curvature (LC) of the aortic arch. Intimal RNA was isolated from the LC and GC of the aortic arch in C57Bl/6 mice and ZBTB46 mRNA expression in the LC was compared to the GC showing downregulation of ZBTB46 in LC. *P < 0.05 one-tailed t-test, n = 7

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