Fig. 2 | Laboratory Investigation

Fig. 2

From: ZBTB46 is a shear-sensitive transcription factor inhibiting endothelial cell proliferation via gene expression regulation of cell cycle proteins

Fig. 2

Expression of ZBTB46 in endotheal cells at both mRNA and protein levels is induced in confluent human aortic cells in vitro and is shear sensitive, being significantly downregulated by low and oscillatory shear stress. a ZBTB46 mRNA expression in HAECs cultured at various densities (20–100%) and up to 6 days post confluence. ***P < 0.001, compared to the 20% confluent group, one-way ANOVA, n = 6. b, c Western blot assay for ZBTB46 protein content in 50% confluent HAECs compared to HAECs cultured 2 days post confluent, quantified using ImageJ. ***P < 0.001, two-tailed t-test, n = 4. d–f HAECs were cultured under unidirectional laminar shear stress (LS, 15 dyn/cm2) or low and oscillatory shear stress (OS, ±5 dyn/cm2) for 2 days using a cone-and-plate in vitro shear system. d ZBTB46 mRNA expression by qPCR. e, f ZBTB46 protein expression by Western blot, quantified using ImageJ. *P < 0.05, ***P < 0.001, n = 6

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