Fig. 3 | Laboratory Investigation

Fig. 3

From: ZBTB46 is a shear-sensitive transcription factor inhibiting endothelial cell proliferation via gene expression regulation of cell cycle proteins

Fig. 3

Adenoviral-mediated overexpression of ZBTB46 results in a significant increase in ZBTB46 mRNA and protein expression in HAECs, leading to significant supression of EC proliferation. HAECs were transfected with adenovirus transducing both ZBTB46 and GFP as a marker for transduction efficiency (ZBTB46-AV) or adenovirus transducing only GFP as control (GFP-AV) at an adenoviral particle to cell ratio of 20:1, and cultured alongside non-treated control (NTC) HAECs. a–c ZBTB46 mRNA measured by qPCR and protein level (Western blot, quantified using ImageJ software) 3 days post infection. ***P < 0.001, compared to GFP control, one-way ANOVA, n = 4–5/group. d Transduced HAECs were seeded at similar sub-confluent density 24 h after infection alongside NTC cells and were counted manually every 24 h for 4 days. **P < 0.01, ***P < 0.001, compared to GFP control, one-way ANOVA. e Manual count of Trypan Blue (TB)-stained cells (as % of total cells) in ZBTB46-transduced HAECs at day 3 showed no significant difference among groups

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