Fig. 5 | Laboratory Investigation

Fig. 5

From: ZBTB46 is a shear-sensitive transcription factor inhibiting endothelial cell proliferation via gene expression regulation of cell cycle proteins

Fig. 5

ZBTB46 downregulation negates the inhibitory role of LS on EC proliferation, while ZBTB46 overexpression inhibits EC proliferation under OS condition. HAECs were cultured on collagen coated slide chambers and exposed to either LS (15 dyn/cm2) or OS (±5 dyn/cm2) conditions for 2 days using the Ibidi shear stress system following treatment with a ZBTB46 siRNA or control siRNA, and b ZBTB46-AV or control-AV. Quantification of Ki67-positive cells as percentage of total EC was performed using ImageJ software, normalized to the control OS condition. *P < 0.05, **P < 0.01, compared to control OS, n = 3–6/group

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