Fig. 6 | Laboratory Investigation

Fig. 6

From: ZBTB46 is a shear-sensitive transcription factor inhibiting endothelial cell proliferation via gene expression regulation of cell cycle proteins

Fig. 6

Full-length but not truncated version of transduced ZBTB46 exhibits nuclear localization and suppresses cell proliferation in HAECs. a Representative images showing immunofluorescent staining of FLAG-tagged full-length ZBTB46 (F-ZBTB46-AV) or FLAG-tagged truncated version ZBTB46 protein (F-shortZBRB46-AV) compared to control lacZ adenovirus-infected cells (lacZ-AV) using an anti-FLAG antibody (red), and DAPI nuclear counter stain (blue). b Adenovirus-infected cells were seeded at similar sub-confluent density in 24-well plates 1 day post infection. Cell numbers were counted manually every day for 4 days. *P < 0.05, **P < 0.01, ***P < 0.001 compared to lacZ-AV, #P < 0.05, ##P < 0.01, ###P < 0.001 compared to F-shortZBTB46-AV, one-way ANOVA, n = 4

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