Fig. 7 | Laboratory Investigation

Fig. 7

From: ZBTB46 is a shear-sensitive transcription factor inhibiting endothelial cell proliferation via gene expression regulation of cell cycle proteins

Fig. 7

ZBTB46 overexpression results in a broad suppression of multiple cell cycle genes and correspondingly a reduction in phosphorylated retinoblastoma (pRb) protein content in HAECs. a Relative mRNA expression of various genes involved in cell cycle regulation in HAECs overexpressing ZBTB46 compared to controls by qPCR, grouped by their predominant known roles in various phases of the cell cycle. b Representative Western blots showing reduced total Rb and pRb protein content in ZBTB46 transducing HAECs compared to control cells. c Quantification of Rb and pRb protein content based on Western blots. d Calculated ratio of pRb to total Rb showed decreased Rb protein phosphorylation. *P < 0.05, ***P < 0.001 compared to GFP-AV, one-way ANOVA, n = 4–6/group

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