Fig. 2
From: BMP-2 restoration aids in recovery from liver fibrosis by attenuating TGF-β1 signaling
Downregulation of BMP-2 in murine fibrotic livers induced by BDL surgery and CCl4 administration. a Gene expression of BMP-2 in normal and fibrotic mouse livers. BMP-2 mRNA levels were measured by RT-qPCR. b Protein expression of BMP-2 in mouse normal and fibrotic livers. Representative images of western blotting (upper) and densitometry data (lower) are shown. c, d Localization and quantification of hepatic BMP-2 and TGF-β1 expression in normal and fibrotic mouse livers. Representative images of immunohistochemical staining are shown (upper). Arrows indicate hepatocytes; arrowheads indicate HSCs. Signal mean intensities in the fibrotic areas were quantified based on 6–10 different fields/slide (lower). Data represent the mean ± SEM of four or five samples per group. *P < 0.05 compared between the indicated groups. Original magnification: × 200
