Fig. 7
From: BMP-2 restoration aids in recovery from liver fibrosis by attenuating TGF-β1 signaling
Effects of BMP-2 on TGF-β1-stimulated proliferation and migration of primary mouse HSCs. Primary HSCs were pretreated with BMP-2 (250 ng/ml) or PBS (vehicle control) for 1 h, followed by TGF-β1 treatment (10 ng/ml) for 48 h. a Effect of BMP-2 pretreatment on TGF-β1-increased HSC proliferation. A WST-1 assay was performed after 48 h of TGF-β1- stimulation. b Effect of BMP-2 pretreatment on TGF-β1-increased HSC migration. The representative images (original magnification × 40) of a wound-healing migration assay were photographed at the indicated time points. c Image analysis data of migration assay at 15 h. All data are expressed as mean ± SEM (n = 4). *P < 0.05 compared between the indicated groups
