Fig. 8
From: BMP-2 restoration aids in recovery from liver fibrosis by attenuating TGF-β1 signaling
Effects of BMP-2 on TGF-β1-induced activation and EMT marker expression in primary mouse HSCs. Primary HSCs were pretreated with BMP-2 (250 ng/ml) or PBS (vehicle control) for 1 h, followed by TGF-β1 treatment (10 ng/ml) for 48 h. a Immunofluorescent staining of α-SMA (red), fibronectin (green), and nuclei (blue) in HSCs (original magnification × 200). b Fluorescence intensity analysis of α-SMA and fibronectin. c Immunofluorescent staining of E-cadherin (red), vimentin (green), and nuclei (blue) in HSCs (original magnification × 200). d Fluorescence intensity analysis of EMT marker expression, E-cadherin and vimentin. The fluorescence intensities were quantified by counting 5–10 different fields per sample. All data are expressed as mean ± SEM (n = 3). *P < 0.05 compared between the indicated groups
