Fig. 3 | Laboratory Investigation

Fig. 3

From: Attenuation of ventilation-induced diaphragm dysfunction through toll-like receptor 4 and nuclear factor-κB in a murine endotoxemia model

Fig. 3

SN50 abrogated endotoxin-augmented mechanical ventilation-induced atrogin-1, MuRF-1, and LC3-II expression in the diaphragm. Western blots were performed using antibodies that recognize atrogin-1 (a), MuRF-1 (b), LC3-II (c), and GAPDH expression from the diaphragms of nonventilated control mice and mice ventilated at a tidal volume of 6 mL/kg or 10 mL/kg for 8 h with or without LPS administration (n = 5 per group). Arbitrary units were expressed as relative atrogin-1, MuRF-1, and LC3-II activation (n = 5 per group). SN-50 2 mg/kg was given intraperitoneally 30 min before ventilation. *P < 0.05 versus the nonventilated control mice with LPS treatment; †P < 0.05 versus all other groups. GAPDH glyceraldehydes-phosphate dehydrogenase, LC3-II light chain 3-II, MuRF-1 muscle ring finger-1

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