Fig. 4 | Laboratory Investigation

Fig. 4

From: Attenuation of ventilation-induced diaphragm dysfunction through toll-like receptor 4 and nuclear factor-κB in a murine endotoxemia model

Fig. 4

Inhibition of endotoxin-augmented mechanical ventilation-mediated diaphragm dysfunction by TLR4 homozygous knockout. MDA (a), protein carbonyl groups (b), total antioxidant capacity (c), SOD (d), BAL fluid MIP-2 (e), BAL fluid IL-6 (f), and Western blots using antibodies that recognize atrogin-1 (g), MuRF-1 (h), LC3-II (i), and GAPDH expression were from the diaphragms of nonventilated control mice and mice ventilated at a tidal volume of 10 mL/kg for 8 h with or without LPS administration (n = 5 per group). Arbitrary units were expressed as relative atrogin-1, MuRF-1, and LC3-II expression (n = 5 per group). *P < 0.05 versus the nonventilated control mice with LPS treatment; †P < 0.05 versus all other groups

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