Fig. 5 | Laboratory Investigation

Fig. 5

From: Interferon-γ mediates the protective effects of soluble receptor for advanced glycation end-product in myocardial ischemia/reperfusion

Fig. 5

Knockdown β5i abolished the inhibiting effects of sRAGE on H/R-induced apoptosis in cultured cardiomyocytes. Neonatal rat cardiomyocytes were infected with adenovirus expressing GFP (Ad-GFP), sRAGE (Ad-sRAGE) or β5i shRNA (Ad-siβ5i) for at least 24 h and then stimulated H/R. a Representative image of TUNEL staining in cardiomyocytes in each group. Magnification: × 20. Scale bar: 50 μm. b Quantification of TUNEL-positive cells in fold-change over the control group. c Representative image of western blot for caspase-3 in cardiomyocytes for each group. d Quantification of the relative expression of caspase-3 in cardiomyocytes. Values are expressed as the mean ± SEM from three independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001

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