Fig. 5 | Laboratory Investigation

Fig. 5

From: Poldip2 knockdown inhibits vascular smooth muscle proliferation and neointima formation by regulating the expression of PCNA and p21

Fig. 5

Poldip2 knockdown normalizes cell cycle markers following vascular injury. Intraluminal injury was induced by insertion of a guide wire into the right femoral artery in mice. Arteries were harvested 2 weeks after surgery and pooled samples from 2–4 animals were processed for protein analysis using Western blotting. In injured vessels, PCNA was reduced (by 29 ± 16%, n = 5, P < 0.05) and p21 was increased (by 74 ± 27%, n = 3, P < 0.05) in arteries from Poldip2+/‒ vs. wild-type mice. Thus, Poldip2 knockdown appears to normalize cell cycle markers affected by neointimal proliferation. Equal loading between lanes was verified using β-actin. Data from injured arteries are representative from 3–5 independent experiments. Two lanes of uninjured arteries from a single experiment are also included for comparison

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