Fig. 6

Poldip2 knockdown decreases proliferation in cultured VSMC. VSMC were transfected with control (siControl) or Poldip2 (siPoldip2) siRNA. A Transfected cells were counted at the time of plating (day 0, not shown) and maintained in serum-free medium or stimulated with 10% calf serum (days 1–5). Data points represent average number of cells in each group ± SEM from 6 independent experiments. In some conditions, the error bar is smaller than the symbol. While control cells multiplied during that time (***P < 0.001 vs. no serum at days 3–5), Poldip2 knockdown abrogated serum-induced proliferation (###P < 0.001 vs. siControl + serum at days 3–5). B Transfected cells were incubated with the nucleoside analog Edu (10 µM) for 1 day in the presence or absence of 10% calf serum. Edu incorporation was measured by fluorescence. Stimulation with serum increased Edu incorporation in both control and Poldip2 knockdown cells (***P < 0.001 vs. no serum). Poldip2 knockdown abrogated Edu incorporation in the absence or presence of serum (###P < 0.001 vs. siControl). Bars represent the mean ± SEM from 3 independent experiments