Fig. 7 | Laboratory Investigation

Fig. 7

From: Poldip2 knockdown inhibits vascular smooth muscle proliferation and neointima formation by regulating the expression of PCNA and p21

Fig. 7

Poldip2 knockdown affects cell cycle markers in cultured VSMCs. VSMCs were transfected with control (siControl) or Poldip2 (siPoldip2) siRNA and processed for Western blotting after the indicated number of days in culture in the presence of 10% calf serum. Membranes were probed for Poldip2 (A), p21 or PCNA (B) using corresponding primary antibodies. Equal loading was verified using β-actin (A) or β-tubulin (B) antibodies. (C, D) Quantification of results from B. At days 2 and 4, p21 and PCNA were significantly upregulated and downregulated, respectively, by siPoldip2 (*P < 0.05 vs. siControl). Bars represent the average ± SEM of data from 5 (C) and 7 (D) independent experiments. The effectiveness of siPoldip2 treatment from one experiment is shown (A)

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