Fig. 8 | Laboratory Investigation

Fig. 8

From: Poldip2 knockdown inhibits vascular smooth muscle proliferation and neointima formation by regulating the expression of PCNA and p21

Fig. 8

Knockdown of p21 rescues the inhibition of cell proliferation induced by siPoldip2. VSMCs were transfected with a pair of control (siControl), Poldip2 (siPoldip2) or p21 (sip21) siRNAs, as indicated. A Transfected cells were grown for 2 days in the presence of 10% calf serum before harvesting for Western blotting. Membranes were probed with p21, Poldip2 or β-actin primary antibodies to verify the effectiveness of siRNA treatments. These blots are representative of 3–4 independent experiments. B Transfected cells were counted after 2 days in the presence of 10% calf serum. Knockdown of Poldip2 reduced proliferation (**P < 0.01 vs. siControl) and sip21 rescued this inhibition (#P < 0.05 vs. siPoldip2 alone). Bars represent the average ± SEM of cell counts from 3 independent experiments

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