Fig. 2
From: PHF20L1 antagonizes SOX2 proteolysis triggered by the MLL1/WDR5 complexes
Inhibiting the activity of the MLL1/WDR5 complexes stabilize SOX2. a SiRNA-mediated knockdown of the components of the MLL1/WDR5 complexes resulted in the accumulation of SOX2. PA-1 cells were transfected with two pairs of specific siRNAs targeting WDR5, RBBP5, ASH2L, or MLL1 respectively for 48 h. Protein changes were examined by specific antibodies as indicated, and Cullin-1 was served as a loading control. b Densitometry measurements illustrating the relative protein level of SOX2 after knockdown of WDR5, RBBP5, ASH2L, or MLL1, which was normalized to Cullin-1. c Forced expression of WDR5 decreased the protein level of SOX2. PA-1 cells were transfected with pMSCV-TAP-WDR5 and pMSCV-TAP (vector) plasmids, respectively. Forty-eight hours post transfection, proteins of interest were detected using specific antibodies. Densitometry measurements illustrated the relative protein level of SOX2, which was normalized to Cullin-1. d The mRNA levels of SOX2 were stable after knocking down of WDR5, RBBP5, ASH2L, or MLL1. The relative mRNA levels were measured by quantitative real-time PCR after PA-1 cells were transfected with indicated siRNAs for 48 h. **p < 0.01, ***p < 0.001. The data were represented as mean ± SD
