Fig. 4
From: PHF20L1 antagonizes SOX2 proteolysis triggered by the MLL1/WDR5 complexes
PHF20L1 antagonizes the degradation of SOX2. a Knockdown of PHF20L1 reduced the protein of SOX2. PA-1 cells were transfected with two specific PHF20L1 siRNAs respectively and then the protein level of SOX2 was western analyzed, densitometry quantified, and plotted, normalizing to Cullin-1. b The mRNA level of SOX2 was negligibly impaired after knocking down PHF20L1. The mRNA level of PHF20L1 and SOX2 were measured by quantitative real-time PCR. Results were normalized to β-actin. c Over-expression of PHF20L1 induced the accumulation of SOX2 protein in PA-1 cells. Cullin-1 was blotted as a loading control. Densitometry measurements illustrated the relative protein level of SOX2. d SOX2 degradation after PHF20L1 knockdown was mediated by 26S proteasome. PA-1 cells were transfected with control (Luc) or PHF20L1 siRNAs for 48 h, and then incubated with MG-132 for 3 h. The relative protein level of SOX2 was densitometry quantified and plotted. a–d **p < 0.01; ***p < 0.001. The data were represented as mean ± SD
