Fig. 6

Blocking the MBT domain of PHF20L1 destabilizes SOX2. a Inhibiting the MBT domain of PHF20L1 with small chemicals UNC1215 and UNC669 destabilized SOX2. PA-1 cells were incubated with UNC1215 or UNC669 for 24 h and then further treated with either DMSO or MG-132 for 4 h. Densitometry measurements illustrating the relative protein levels of SOX2 and PHF20L1, normalized to Cullin-1, were plotted (right panels). b UNC1215 and UNC669 treatments did not affect the mRNA level of SOX2. Quantitative real-time PCR was performed to measure the mRNA level of SOX2 upon UNC1215 or UNC669 treatments. Results were normalized to β-actin. c Treatment with UNC1215 or UNC669 shortened the half-life of SOX2 protein. PA-1 cells were incubated with 40 μM UNC1215, UNC669, or DMSO for 24 h, and followed by CHX treatment as indicated. The relative protein levels of SOX2 and PHF20L1 were densitometry quantified using Gel Image analysis software and plotted. The data were represented as mean ± SD