Fig. 2
From: The direct binding of collagen XVII and collagen IV is disrupted by pemphigoid autoantibodies
mAb targeting C-terminus (C17-C1) did not co-precipitate COL4. a Schema of full-length human COL17 and recombinant C-terminal-deleted COL17 (COL17Δ1, COL17Δ2) expressed by HEK293 cells. The epitopes of mAb TS39-3 (Asp552-Gln545) and mAb C17-C1 (Gly1316-Asp1340) are indicated. b Immunoprecipitation with mAbs TS39-3, C17-C1, anti-human COL4A1 Ab, or mIgG1 using cell lysates of NHOMK. Blotting was performed using anti-human COL17 Ab or anti-human COL4A1 Ab. The protein amounts in the cell lysates were analyzed by immunoblotting as input (10% amount of IP). c Immunoprecipitation with mAbs TS39-3, C17-C1, or mIgG1 using cell lysates of NHOMK. d Immunoblotting was performed using anti-human laminin 332, anti-human integrin α6, or anti-human integrin β4. IP (Ab) immunoprecipitation antibody, IB (Ab) immunoblotting antibody
