Fig. 4 | Laboratory Investigation

Fig. 4

From: Vitamin D protects human melanocytes against oxidative damage by activation of Wnt/β-catenin signaling

Fig. 4

β-catenin silencing abolished the protection of vitamin D in H2O2-treated melanocytes. Quantitative real-time PCR (a) and Western blotting (b) were carried out to evaluate β-catenin expression in melanocytes transfected with β-catenin siRNA and an irrelevant siRNA control. BC blank control, NC negative control, S1-S3 β-catenin siRNA#1-#3. c Measurement of intracellular ROS. d Cell viability determination by the CCK-8 assay. e Cell migration assay. f Cell apoptosis assay. *P  <  0.05, **P  <  0.01, ***P < 0.001, compared with the control group; #P < 0.05, ##P < 0.01, ###P < 0.001, compared with the H2O2 group

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