Fig. 3 | Laboratory Investigation

Fig. 3

From: The role of Ca2+ in acid-sensing ion channel 1a-mediated chondrocyte pyroptosis in rat adjuvant arthritis

Fig. 3

Effects of extracellular acidosis on pyroptosis in primary articular chondrocytes. a The effects of treatment with several pH levels on the protein levels of ASC, NLRP3, cleaved caspase-1p10 and ASIC1a. b The effects of pH 6.0 stimulation on the protein levels of ASC, NLRP3, cleaved caspase-1p10 and ASIC1a. c Real-time PCR analysis of ASC, NLRP3 and caspase-1 in primary articular chondrocytes. d Extracellular acidosis induced the expression of IL-1β and IL-18 in culture medium by ELISA. e Extracellular acidosis induced the mRNA expression of IL-1β and IL-18. The data are presented as the mean ± SEM of three wells in three independent experiments, *P < 0.05, **P < 0.01 vs. pH 7.4 group

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