Fig. 4

Effects of extracellular acidosis on pyroptosis in primary articular chondrocytes and the construction of the ASIC1a knock-down model. a Extracellular acidosis induced the expression of ASC and NLRP3 by immunofluorescence. b Extracellular acidosis induced the release of LDH. c Photographs of chondrocytes after transfection with ASIC1a shRNA; d, e. ASIC1a shRNA inhibits the mRNA and protein expression of ASIC1a. The data are presented as the mean ± SEM of three wells in three independent experiments, ^*P < 0.05, ^**P < 0.01 vs. pH 7.4 group; ^#P < 0.05, ^##P < 0.01 vs. pH 6.0 group