Fig. 5

Activation of ASIC1a promoted extracellular acidosis-induced pyroptosis in primary articular chondrocytes. a ASIC1a-specific inhibitor Pctx-1 and ASIC1a shRNA attenuated extracellular acidosis-induced pyroptosis in primary articular chondrocytes by western blotting for ASC, NLRP3 and cleaved caspase-1p10. b The negative effects of Pctx-1 and ASIC1a shRNA on extracellular acidosis-induced pyroptosis in primary articular chondrocytes. c Pctx-1 and ASIC1a shRNA attenuated the levels of IL-1β and IL-18 in culture medium. d Pctx-1 suppressed the release of LDH in primary articular chondrocytes. The data are presented as the mean ± SEM of three wells in three independent experiments, ^*P < 0.05, ^**P < 0.01 vs. pH 7.4 group; ^#P < 0.05, ^##P < 0.01 vs. pH 6.0 group