Fig. 6
Activation of ASIC1a promoted extracellular acidosis-induced pyroptosis in primary articular chondrocytes and the upregulation of intracellular [Ca2 + ]i. a Pctx-1 inhibited pyroptosis induced by extracellular acidosis in primary articular chondrocytes by AO/EB staining, semi-quantitative results. b Pctx-1 downregulated the expression of ASC and NLRP3 in primary articular chondrocytes by immunofluorescence. c Pctx-1 downregulates intracellular [Ca2+]i by laser scanning confocal microscopy. The data are presented as the mean ± SEM of three wells in three independent experiments, *P < 0.05, **P < 0.01 vs. pH 7.4 group; #P < 0.05, ##P < 0.01 vs. pH 6.0 group
