Table 1 Primer sequences of several genes for real-time PCR
Gene | Sequences | Length (bp) |
|---|---|---|
ASC | F:5′-TTATGGAAGAGTCTGGAGCTGTGG-3′ | 101 |
R:5′-AATGAGTGCTTGCCTGTGTTGG-3′ | ||
NLRP3 | F:5′-CAGACCTCCAAGACCACGACTG-3′ | 127 |
R:5′-CATCCGCAGCCAATGAACAGAG-3′ | ||
Caspase-1 | F:5′-TGCCTGGTCTTGTGACTTGGAG-3′ | 133 |
R:5′-ATGTCCTGGGAAGAGGTAGAAACG-3′ | ||
IL-1β | F:5′-CTCAACTGTGAAATAGCAGCTTTC-3′ | 110 |
R:5′-GGACAGCCCAAGTCAAGG-3′ | ||
IL-18 | F: 5′-ATATCGACCGAACAGCCAAC-3′ | 105 |
R:5′-TTCCATCCTTCACAGATAGGG-3′ | ||
ASIC1a | R:5′-GGCCAACTTCCGTAGCTTCA-3′ | 67 |
R:5′-ATGCCCTGCTCTGTCGTAGAA-3′ | ||
β-actin | F:5′-GGAGATTACTGCCCTGGCTCCTA-3′ | 150 |
R:5′-GACTCATCGTACTCCTGCTTGCTG-3′ |
