Fig. 5 | Laboratory Investigation

Fig. 5

From: Mitochondrial pyruvate carrier 1 functions as a tumor suppressor and predicts the prognosis of human renal cell carcinoma

Fig. 5

Involvement of MPC1 in the migration and invasion of RCC cells through regulation of HIF1α. a The expression of MPC1 in 786-O cells treated with 200 μM CoCl2 in normal (20% O2) or hypoxia conditions (1% O2). b Western blotting of MPC1 and HIF1α in 786-O cells transfected with siCtrl and siMPC1. GAPDH serves as a loading control. c The expression of HIF1α in Mock and OE-MPC1 cells detected by Western blotting. GAPDH serves as a control. d Representative IHC staining for MPC1 and HIF1α in RCC tissues. Black arrows: cancer cells. e χ2 analysis of the percentage of HIF1αhigh and HIF1αlow cells in MPC1low and MPC1high RCC specimens (n = 150)

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