Fig. 6 | Laboratory Investigation

Fig. 6

From: MicroRNA amplification and detection technologies: opportunities and challenges for point of care diagnostics

Fig. 6

Microfluidic chip-based approaches. a The power-free microfluidic device in which PDMS absorbs air in the outlet chamber thus being a self-stand pumping device. Probe DNA is immobilized onto the glass surface, and microchannels convey the sample to the probe and miRNA hybridization and detection takes place. b An enlarged view of a laminar flow in the microchannel. The laminar flow conveys F-SA and B-anti-SA. Sandwich hybridization and dendritic amplification take place at the intersection between the probe DNA-patterned surface and the interface of the laminar flow. F-SA FITC-labeled streptavidin, B-anti-SA biotinylated anti-streptavidin. Reproduced with permission from refs. [90, 91]

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