Fig. 2 | Laboratory Investigation

Fig. 2

From: Osteoblast lineage-specific cell-surface antigen (A7) regulates osteoclast recruitment and calcification during bone remodeling

Fig. 2

Expression pattern of A7 antigen in osteoblastic cell line observed by two and three-dimensional confocal microscope imaging. ROS17/2.8 CL#7 cells were cultured and stained with A7 MAb as described in the legends of Fig. 1. a Expression and distribution pattern of A7 antigen detected by A7 MAb in a horizontal section of ROS17/2.8 CL#7 cells parallel to the bottom of the culture dish. Bars, 10 μm. b–d Three dimensional images of A7 antigen expression detected by A7 MAb in ROS17/2.8 CL#7 cells from different views (A7 MAb/DAPI merge). Three-dimensional images were reconstructed from confocal images of each height shown in supplemental Fig. 1. b Top view showing A7 antigen expression on apical and lateral cell surfaces. c Bottom view showing the basal cell surface attaching the culture dish. d Top and side view. Bars, 10 µm

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