Fig. 9 | Laboratory Investigation

Fig. 9

From: Osteoblast lineage-specific cell-surface antigen (A7) regulates osteoclast recruitment and calcification during bone remodeling

Fig. 9

Cross-linking of A7 antigen stimulates osteoclastogenesis Bone marrow cells of 4-week-old SD male rats were cultured in 96 multi-well plates (0.2 × 106 cells/well) to induce osteoclasts as described in Materials and Methods (hybridoma screening) in the presence of various concentrations of A7 MAb or isotype control IgG. Cells were fixed and stained for TRAP at day 4 of culture. TRAP-positive cells having more than three nuclei were counted as osteoclasts. a Significant enhancement of osteoclastogenesis by A7 MAb in cultures of moderate osteoclast formation. b Significant up-regulation of osteoclastogenesis by A7 MAb in cultures of abundant osteoclast formation. Data represent mean ± SEM (n = 6). c Absence of any modulatory effects on osteoclastogenesis by isotype control IgG1. *p < 0.05, significant if compared to the control cultures containing no antibody

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