Table 2 Stereological parameters measured at electron microscopic level

From: Surfactant dysfunction and alveolar collapse are linked with fibrotic septal wall remodeling in the TGF-β1-induced mouse model of pulmonary fibrosis

Parameter

Determination

Primary magnification

Volume of alveolar epithelial type 1 cells in septal walls V(AE1,sep)

Point counting

× 11.000

Volume of alveolar epithelial type 2 cells in septal walls V (AE2,sep)

Point counting

× 11.000

Volume of collagen fibrils within septal walls V(col,sep)

Point counting

× 11.000

Volume of the residual extra- cellular matrix within septal walls V(rECM,sep)

Point counting

× 11.000

Volume of interstitial cells within septal walls V(IC,sep)

Point counting

× 11.000

Volume of capillary lumen within septal walls V(caplumen,sep)

Point counting

× 11.000

Volume of endothelial cells within septal walls V(endo,sep)

Point counting

× 11.000

Surface area of capillary endothelia within septal walls S(cap,sep)

Combined point-/ intersection counting

× 11.000

Volume of lamellar bodies within the lung V(lb,lung)

Point counting

× 8.900

Surface area of apical membrane of AE2 cells S(apicalAE2,lung)

Combined point-/ intersection counting

× 8.900

Surface area of basolateral membrane of AE2 cells S(blAE2,lung)

Combined point-/ intersection counting

× 8.900

Apical/basal ratio

S(apicalAE2,lung)/S(blAE2,lung)