Table 2 Stereological parameters measured at electron microscopic level
Parameter | Determination | Primary magnification |
---|---|---|
Volume of alveolar epithelial type 1 cells in septal walls V(AE1,sep) | Point counting | × 11.000 |
Volume of alveolar epithelial type 2 cells in septal walls V (AE2,sep) | Point counting | × 11.000 |
Volume of collagen fibrils within septal walls V(col,sep) | Point counting | × 11.000 |
Volume of the residual extra- cellular matrix within septal walls V(rECM,sep) | Point counting | × 11.000 |
Volume of interstitial cells within septal walls V(IC,sep) | Point counting | × 11.000 |
Volume of capillary lumen within septal walls V(caplumen,sep) | Point counting | × 11.000 |
Volume of endothelial cells within septal walls V(endo,sep) | Point counting | × 11.000 |
Surface area of capillary endothelia within septal walls S(cap,sep) | Combined point-/ intersection counting | × 11.000 |
Volume of lamellar bodies within the lung V(lb,lung) | Point counting | × 8.900 |
Surface area of apical membrane of AE2 cells S(apicalAE2,lung) | Combined point-/ intersection counting | × 8.900 |
Surface area of basolateral membrane of AE2 cells S(blAE2,lung) | Combined point-/ intersection counting | × 8.900 |
Apical/basal ratio | S(apicalAE2,lung)/S(blAE2,lung) |