Fig. 5 | Laboratory Investigation

Fig. 5

From: Endotoxemia-induced endothelial fibrosis inhibition improves hypotension, tachycardia, multiple organ dysfunction syndrome, cytokine response, oxidative stress, and survival

Fig. 5

GW-induced endothelial fibrosis inhibition decreases liver and kidney tissue fibrosis in 24-h endotoxemic rats. Representative images of picroSirius red stained liver (a–d) and kidney (f–l) sections from vehicle-treated/saline-treated rats (a, f), vehicle-treated/endotoxemic rats (b, g), GW-treated/endotoxemic rats (c, h) and GW-treated/saline-treated rats (d, i), after 24 h. GW-788388 was administrated by gavage (5 mg/kg a day) 24 h before and during 24-h endotoxemia. Images were subjected to analysis of tissue fibrosis in liver (e) and kidney (glomerular, j and interstitial, k). Values are expressed as the mean ± SD. *p < 0.05, **p < 0.01, assessed by one-way ANOVA (Kruskal–Wallis) and Dunn’s post-test. N = 8. Representative images (l, n, p, r) and densitometric analyses (m, o, q, s) from western blot experiments performed for the detection of collagen III (l, m, p, q) and fibronectin (n, o, r, s) protein levels in liver (l, m, n, o) and kidney (p, q, r, s). Values are expressed as the mean ± SD. **p < 0.01, assessed by one-way ANOVA (Kruskal–Wallis) and Dunn’s post-test. N = 6

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