Fig. 5 | Laboratory Investigation

Fig. 5

From: N-terminal sequences in matrin 3 mediate phase separation into droplet-like structures that recruit TDP43 variants lacking RNA binding elements

Fig. 5

Colocalization of MATR3:YFP-WT-ΔRRM2 and TDP43:mCherry variants with disabled RRM1 domains. Mouse C2C12 cells were cotransfected with MATR3:YFP and TDP43:mCher constructs as indicated below. The images shown are representative of what was found in at least two separate transfection experiments analyzing 50–100 transfected cells in each experiment. a−d Images were captured with a Nikon A1RMP imaging system (original magnification ×60 with ×2 digital enlargement; scale bars are approximations). Each image is from a single z-plane. a, b WT MATR3:YFPΔRRM coexpressed with TDP43:mCherΔRRM1 or TDP43:mCherRRM1-M. c, d F115C or S85C MATR3:YFPΔRRM coexpressed with TDP43:mCherRRM1-M. e WT MATR3:YFPΔRRM coexpressed with WT TDP43:mCher at original magnification (×60). Expression plasmids were generated as described in Materials and methods

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