Fig. 6 | Laboratory Investigation

Fig. 6

From: N-terminal sequences in matrin 3 mediate phase separation into droplet-like structures that recruit TDP43 variants lacking RNA binding elements

Fig. 6

Colocalization of NLS-N397:MATR3WT:YFP with PrL domain of TDP43 in spherical droplet-like structures. a, b Mouse C2C12 cells were grown on glass coverslips and transiently cotransfected with pEF.Bos vectors encoding NLS-N397-MATR3:YFP and NLS-C263-TDP43:mCher; a shows the YFP channel and b shows the mCherry channel. c Cells transfected with NLS-C263-TDP43:mCher alone. d, e Mouse C2C12 cells cotransfected with NLS-N397-MATR3:YFP and NLS-N284-FUS:mCher; d shows the YFP channel and e shows the mCher channel. f Cells transfected with NLS-N284-FUS:mCher, alone. At 24 h posttransfection, the cells were fixed and imaged with an Olympus spinning disk confocal microscope (projection images; original magnification ×40). The images shown are representative of images seen in three independent transfections, viewing at least 50 cells on each cover slip

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