Fig. 2
LDVmax and Dmax are increased in aggressive cancer cells and they depend on an intact MT-network. LDs were stained with BODIPY and a their maximum velocity (LDVmax) was calculated in NHPrE1, LNCaP, and PC-3 cells, breast benign epithelial cells (MCF10A), nonmetastatic (MCF-7) and metastatic (MDA) cancer cells, and in pancreatic (PANC-1) cancer cells. b The maximum displacement (Dmax), defined by the longest vector of displacement from the point of origin in a track, was assessed in NHPrE1, LNCaP and PC-3 cells. c The proportion of directional movement was evaluated in benign and PCa cells. d Nondirectional movement of an LD in a PCa cell as indicated by the nonlinear trajectory (black arrow: starting point; red arrow: ending point). e Directional movement of an LD in a PCa cell up to for ~2/3 of its length (asterisk) as represented by the linear trajectory (black arrow: starting point; red arrow: ending point). f Nondirectional movement was assessed using a non-quadratic mean square displacement (MSD) plot. g Directional movement was assessed using a quadratic MSD. n = 50. Values are mean ± SEM. Student’s unpaired t-test. **P < 0.01, ***P < 0.001
