Fig. 1

TRPV4 gene is not involved in airway hyperresponsiveness. a Graphical representation of chicken ovalbumin-based airway allergy model. C57BL/6 WT and TRPV4 KO mice (6–8-week old) were sensitized and challenged with OVA allergen. On day zero, mice were sensitized with 100 μg OVA combined with 4 mg of Alum via intraperitoneal injection. On day 7 and 14, 100 μg of OVA in PBS was given intraperitoneally. From day 21 to 26, the mice were challenged with nebulized 1% OVA in PBS. AHR was measured 24 h (day 27) after the last OVA challenge, and on day 28, the mice were euthanized and the lung, BAL, and blood were collected for further analysis. b TRPV4 KO and wild-type mice were sensitized and challenged with OVA. Baseline AHR was measured 24 h after the last OVA challenge and following 2 min exposure to PBS (vehicle control) or incremental doses of β-methacholine in PBS (3–100 mg/ml). Airway resistance is expressed as PenH. Data are mean ± SEM of four individual mice