Fig. 5

HMGB1 mediates LPS+CS-induced RAW 264.7 cell death. RAW 264.7 cells were transfected with 100 nM of control siRNA or HMGB1-specific siRNA for 48 h and then subjected to 1 μg/ml LPS for 24 h plus 24% elongation stretch for 12 h at a frequency of 30 cycles/min. a Western blot analysis of HMGB1 knockdown in RAW 264.7 cells. b Western blot analysis of the levels of LC3-I, LC3-II in RAW 264.7 cells treated with LPS+CS, or/and HMGB1 siRNA or scramble (Ctr) siRNA. c Western blot analysis of the levels of cleaved caspase 3 and total caspase 3 in RAW 264.7 cells treated with LPS+CS or/and HMGB1 siRNA or scramble (Ctr) siRNA. d Measurement of the released LDH in RAW 264.7 cells treated with LPS+CS or/and HMGB1 siRNA or scramble (Ctr) siRNA. Data represented the mean ± SEM from at least three independent experiments. *p < 0.05 and **p < 0.01, compared with control or Ctr siRNA+LPS+CS