Fig. 1 | Laboratory Investigation

Fig. 1

From: The Shh/Gli signaling cascade regulates myofibroblastic activation of lung-resident mesenchymal stem cells via the modulation of Wnt10a expression during pulmonary fibrogenesis

Fig. 1

Wnt10a is induced in the bleomycin mouse model. Mice (n = 6 per group) received either saline or bleomycin (BLM) (5 mg/kg body weight) intratracheally and were killed 7, 14, or 21 days (d) later and their lung tissues were analyzed. a Pulmonary fibrotic lesions were determined by hematoxylin-eosin (H&E) and Masson’s trichrome staining. b The mRNA levels of 19 Wnt genes were determined by qRT-PCR. The results are shown as the means ± SD (*P < 0.05 versus control). c The expression of fibronectin, collagen I, α-smooth muscle actin (α-SMA), and Wnt10a was measured by western blot. d Wnt10a expression in lung sections from control and BLM-treated mice was analyzed by immunohistochemistry. e The percentages of Wnt10a+ areas were quantified (*P < 0.05 versus control). f Cytoplasmic and nuclear extracts were prepared and analyzed by western blot using anti-p-β-catenin and anti-β-catenin antibodies. Lamin B and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were used as the loading controls for nuclear and cytoplasmic proteins, respectively

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