Fig. 6 | Laboratory Investigation

Fig. 6

From: The Shh/Gli signaling cascade regulates myofibroblastic activation of lung-resident mesenchymal stem cells via the modulation of Wnt10a expression during pulmonary fibrogenesis

Fig. 6

Wnt10a overexpression and sonic hedgehog (Shh) pathway activation are found in human pulmonary fibrosis. a Wnt10a expression in lung sections from healthy subjects and patients with idiopathic pulmonary fibrosis (IPF) was analyzed using immunohistochemistry. The percentages of Wnt10a+ areas in human lung tissues were quantified (*P < 0.05 versus normal). b The expression of α-smooth muscle actin (α-SMA) in stem cell antigen-1 (Sca-1)+ human lung-resident mesenchymal stem cells (LR-MSCs) was assessed by immunofluorescence. c Colocalization of Wnt10a and Sca-1 or α-SMA in human lung tissues was examined by confocal fluorescence microscopy. Human LR-MSCs were treated with 10 ng/ml human transforming growth factor-β1 (hTGF-β1) for 48 h followed by the measurement of the expression of α-SMA and Wnt10a using immunofluorescence (d) and the measurement of the mRNA levels of Wnt10a (e) and hedgehog pathway components (f) using qRT-PCR. The results are shown as the means ± SD (*P < 0.05 versus control)

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