Fig. 2 | Laboratory Investigation

Fig. 2

From: CCL8 secreted by tumor-associated macrophages promotes invasion and stemness of glioblastoma cells via ERK1/2 signaling

Fig. 2

CCL8 promotes growth and invasion of glioma in vivo. a, b GBM1 cells with or without CCL8 were transplanted subcutaneously into the hips of NOD-SCID mice. CCL8 or PBS was then intratumorally injected at the day 7th and 14th post transplantation, and tumor volume was measured (a). b mice were sacrificed at the 28th day and tumors were weighted. c, d CCL8 shorts the survival time and increases tumor invasion in GL-261 orthotopical mice model. GL-261 cells with CCL8 (5 ng/μL) or PBS were coinjected into C57/B6 mice, and the survival time of mice were analyzed by Kaplan–Meier survival analysis (c). d At the day 12th mice were sacrificed and the invasive sites of tumor were microscopically observed and calculated. e GBM1 cells were orthotopically transplanted into NOD-SCID mice and tumor morphology was observed at day 12th

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