Fig. 2: Accumulation of CD4⁺ and CD11b⁺ cells in diaphragm tissue after LPS injection.

a Representative images showing immunofluorescence staining of CD4 (red) or CD11b (green) in diaphragm tissue from WT and RAMP1^−/− mice at day 3. Nuclei are stained by DAPI (blue). Scale bars, 100 μm. b Number of CD4⁺ cells after LPS injection. Data are expressed as the mean ± SEM (4–6 mice per group). *p < 0.05. c Number of CD11b⁺ cells after LPS injection. Data are expressed as the mean ± SEM (4–6 mice per group). *p < 0.05. d Representative images showing dual immunofluorescence labeling of CD4 (red) and VEGF-C (green) or VEGF-D (red) in tissue from WT mice at day 3. Nuclei are stained by DAPI (blue). Merged signals are yellow. Scale bars, 50 μm. e Representative images of diaphragm tissue from WT mice showing immunofluorescence staining of tissues for CD11b (red) and VEGF-C (green) or VEGF-D (red) (day 3). Nuclei are stained by DAPI (blue). Merged signals are yellow. Scale bars, 50 μm.