Fig. 3: SOX2 knockdown experiments in the SCLC cell lines.
From: Distinct transcriptional programs of SOX2 in different types of small cell lung cancers
a SOX2 affects INSM1, ASCL1, WNT11, and CDH1 expression in SCLC cell lines. The suppression of INSM1, ASCL1, WNT11, CDH1, and p-HH3 was observed in the SCLC-A subtype cell lines (H69, H889, and SBC1s) with RNAi for SOX2. b SOX2 was involved in the expression of NOTCH1, MYC, TCF4, Trp53, and RBL1 in ASCL1-TF A549 cells. We also evaluated the changes of mRNA expression levels corresponding to the proteins that were altered following SOX2 knockdown in each cell line by qRT-PCR. c Cell counting assays with SCLC cell lines. The cell growth curve is shown. The suppression of cell proliferation was observed in H69, H889, and SBC1 cells with RNAi against SOX2. The analysis was performed in triplicate. Data are shown as the mean ± SD. Asterisks indicate a significant difference; *p < 0.05.
